Evaluation of the Effect of Daptomycin, a Glycopeptide Agent, on Intact Intervertebral Disc Tissue

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Date
2019-02-28Author
Kaplan, Necati
Yılmaz, İbrahim
Karaarslan, Numan
Şirin, Duygu Yaşar
Şimşek, Abdullah Talha
Çalışkan, Tezcan
Bircan, Rıfat
Özbek, Hanefi
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AIM: To evaluate the effects of pre- and intra-operatively administered daptomycin (DAP) on the intact human primary intervertebral
disc tissue cells.
MATERIAL and METHODS: Primary cell cultures were established using tissues obtained through decompressive laminectomy,
traumatic intervertebral disc herniation excision, and posterior transpedicular stabilization. Non-drug-administered samples were
used as a control group. The samples treated with DAP formed the study group. Molecular assays for proliferation and gene
expression were performed. The obtained data were evaluated statistically, and results with a value of p<0.05 were accepted as
significant.
RESULTS: While no reduction was observed in the proliferation, the gene expression of intact intervertebral disc tissue cells was
time-dependently decreased compared to the control group, and these results were reported to be statistically significant.
CONCLUSION: This study observed the effect that a pharmaceutical preparation, which was used on intervertebral disc tissue
before and after the operation, had on normal, healthy, and intact tissue. It concludes that alterations in the expression of genes
involved in the anabolic and/or catabolic process, even in adjacent healthy tissue, may slow down the healing process of the
damaged tissue or cause undesired cell differentiation.
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