Does Nimodipine, a Selective Calcium Channel Blocker, Impair Chondro-cyte Proliferation or Damage Extracellular Matrix Structures?
Date
2019-04-17Author
Kaplan, Necati
Yılmaz, İbrahim
Karaarslan, Numan
Kaya, Yasin Emre
Şirin, Duygu Yaşar
Özbek, Hanefi
Metadata
Show full item recordAbstract
Background:
The study aimed to investigate the effects of the active ingredient, nimodipine, on chondrocyte proliferation and extracellular matrix (ECM) structures in cartilage tissue cells.
Methods:
Chondrocyte cultures were prepared from tissues resected via surgical operations. Nimodipine was then applied to these cultures and molecular analysis was performed. The data obtained were statisti-cally calculated.
Results:
Both, the results of the (3-(4,5 dimethylthiazol2-yl)-2,5-diphenyltetrazolium (MTT) assay and the fluorescence microscope analysis [a membrane permeability test carried out with acridine or-ange/propidium iodide staining (AO/PI)] confirmed that the active ingredient, nimodipine, negatively af-fects the cell cultures.
Conclusion:
Nimodipine was reported to suppress cellular proliferation; chondroadherin (CHAD) and hypoxia-inducible factor-1 alpha (HIF-1α) expression thus decreased by 2.4 and 1.7 times, respectively, at 24 hrs when compared to the control group (p < 0.05). Furthermore, type II collagen (COL2A1) ex-pression was not detected (p < 0.05). The risk that a drug prescribed by a clinician in an innocuous man-ner to treat a patient by relieving the symptoms of a disease may affect the proliferation, differentiation, and viability of other cells and/or tissues at the molecular level, beyond its known side effects or adverse events, should not be forgotten.
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