Investigation of the Effects of Methylphenidate, an Amphetamine Derivative, on Intervertebral Disc Tissue Cell Cultures and Matrix Structures
Date
2019-07-24Author
Kaya, Yasin Emre
Karaarslan, Numan
Şirin, Duygu Yaşar
Özbek, Hanefi
Kaplan, Necati
Yılmaz, İbrahim
Metadata
Show full item recordAbstract
AIM: To investigate the effects of methylphenidate (MPH), on intervertebral disc tissue (IVD) cell cultures and extracellular matrix
structures. Changes in the expression of some important marker genes involved in anabolic and catabolic mechanisms of IVD
extracellular matrix formation were also investigated.
MATERIAL and METHODS: Primary cultures of nucleus pulposus cells (NPCs) and annulus fibrosus cells (AFCs) were isolated from
tissues obtained from the operated patients. Cell viability and proliferation were tested, and the cell surface morphologies were
evaluated by microscopy. The expressions of the chondroadherin (CHAD), cartilage oligomeric matrix protein (COMP), interleukin-1
beta (IL-1β) and matrix metalloproteinase (MMP) -7 and MMP-19 genes were evaluated using the quantitative real-time polymerase
chain reaction (qRT-PCR). A value of p<0.05 was considered statistically significant.
RESULTS: The viability and proliferation of intervertebral disc tissue cells decreased in response to MPH treatment and the
expression of the investigated genes also changed.
CONCLUSION: The data obtained from in-vitro studies may not directly adaptable to clinical applications. However, the fact that
the central nervous system stimulant MPH can suppress proliferation of cells derived from IVD tissue should be considered carefully
by clinicians.
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