Are Intervertebral Disc Tissue Cells Damaged When Attempting to Prevent Thrombus Formation Using Dabigatran, A New Oral Anticoagulant?
Date
2019-05-22Author
Kaplan, Necati
Karaarslan, Numan
Yılmaz, İbrahim
Şirin, Duygu Yaşar
Akgün, Feride Sinem
Çalışkan, Tazcan
Şimşek, Abdullah Talha
Özbek, Hanefi
Metadata
Show full item recordAbstract
AIM: To investigate the effect of dabigatran, a new oral anticoagulant, on human primary cell cultures isolated from intact
intervertebral disc tissue.
MATERIAL and METHODS: Cell cultures were prepared from tissues obtained from six cases who had undergone surgery due
to spinal trauma. Dabigatran, an active pharmacological agent, was applied to intact annulus fibrosus (AF)/nucleus pulposus (NP)
primary cell cultures from the study group. After performing cell viability, toxicity, and proliferation tests on all cultures in the control
and study groups, the surface morphologies of the samples were evaluated. Subsequently, chondroadherin (CHAD), cartilage
oligomeric matrix protein (COMP), and matrix metalloproteinase (MMP)-13 and -19 expressions were measured via a real-time
polymerase chain reaction (RT-PCR). Data were analyzed statistically.
RESULTS: In the proliferation assays performed on the 20th day of the study, cells in the dabigatran-supplemented group were
reported to have lost 46.37% more viability than those in the control group. Expressions of all genes examined except MMP-13 were
evaluated in the control group by time, but in contrast to the control group results, COMP and MMP-19 gene expressions decreased
in the dabigatran-treated group. No CHAD or MMP-13 expression was noted in these cultures.
CONCLUSION: The potential for a systemically applied drug to accumulate in tissue and negatively affect surrounding tissues and
microstructures must be emphasized.
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