Are radio‐contrast agents commonly used in discography toxicto the intact intervertebral disc tissue cells?
Date
2018-08-13Author
Karaarslan, Numan
Yılmaz, İbrahim
Özbek, Hanefi
şirin, Duygu Yaşar
Kaplan, Necati
Çalışkan, Tezcan
Özdemir, Çiğdem
Akyuva, Yener
Ateş, Özkan
Metadata
Show full item recordAbstract
In the literature, there have been no studies showing clear results on how radio‐contrast pharmaceuticals would affect intact disc tissue cells. In this context, itwas aimed to evaluate the effects of iopromide and gadoxetic acid, frequentlyused in the discography, on intact lumbar disc tissue in pharmaco‐molecular andhistopathological level. Primary cell cultures were prepared from the healthy disctissue of the patients operated in the neurosurgery clinic. Except for the controlgroup, the cultures were incubated with the indicated radio‐contrast agents. Cellviability, toxicity and proliferation indices were tested at specific time intervals.The cell viability was quantitatively analysed. It was also visually recheckedunder a fluorescence microscope with acridine orange/propidium iodide staining.Simultaneously, cell surface morphology was analysed with an inverted lightmicroscope, while haematoxylin and eosin (H&E) staining methodology was usedin the histopathological evaluations. The obtained data were evaluated statistically.Unlike the literature, iopromide or gadoxetic acid did not have any adverse effectson the cell viability, proliferation and toxicity (P< 0.05). Although this studyreveals that radio‐contrast pharmaceuticals used in the discography, often used inneurosurgical practice, can be safely used, it should be remembered that this study was performed in an in vitro environment.
Collections
- Radyoterapi [25]