Which pharmacological agents may be used to establish healthy, proliferating human primer nucleus pulposus / annulus fibrosus cell cultures? Systematic evaluation of our experience in the light of literature
Date
2018-03Author
Karaarslan, Numan
Yılmaz, İbrahim
Akgün, Feride Sinem
Çalışkan, Tezcan
Şimşek, Abdullah Talha
Kaplan, Necati
Kaya, Yasin Emre
Şirin, Duygu Yaşar
Özbek, Hanefi
Ateş, Özkan
Metadata
Show full item recordAbstract
Intervertebral disc tissue is damaged and loses its normal physiological
functions due to age-related degeneration, vigorous physical activity and
trauma in all age groups and at any stage of life. Here we aim to present how
to prepare human primary cell cultures effectively from intact intervertebral
disc tissues obtained from the patients undergoing operations for spinal
trauma and, from degenerated disc tissues obtained from patients with a
lumbar herniated. In addition, it is aimed to explain bio/pharmaco-molecular
methods used in their analyses at a molecular level in line with the current
literature. Articles included in Pubmed between June 1988 and January 31,
2018, were searched with the key words “nucleus pulposus cell
proliferation” and/or “annulus fibrosus” without language restriction. Data
of descriptive statistics were analyzed using mean ± standard deviation or
frequency (%) and demonstrated using Microsoft Office 2010 and Excel. In
addition, it was explained how to obtain human primary cell culture from the
tissues taken from two different localizations of the single intervertebral
disc. A total of 419 articles were found to be potentially relevant, as a result
of first search. In this literature review, cell cultures, which could possibly
be the main focus of cell therapies in the field of neurosurgery in the near
future, as well as current data were discussed from a molecular point of
view
Collections
- Radyoterapi [25]